Effect of synthesized dipeptide L-Leu-Lys on cell proliferation and apoptosis in organotypic tissue culture from rat spleen
نویسنده
چکیده
The effect of first synthesized dipeptide L-Leu-Lys was studied on cell proliferation and apoptosis using the organotypic culture of spleen tissue from 3-mont-old rats. The dipeptide at concentrations in diapason 0.001 -10 ng/ml produce a clearly defined stimulating effect on cell proliferation in these tissues, accompanying by an increase of expression of Ki67 and decrease of P53 expression. One can suppose that this dipeptide with high biological activity create the base for the further experiments in laboratory animals and clinical trials for investigation of their effect on the immune system. 2015 Trade Science Inc. INDIA
منابع مشابه
Effect of Chemotherapeutic Drugs on Caspase-3 Activity, as a Key Biomarker for Apoptosis in Ovarian Tumor Cell Cultured as Monolayer. A Pilot Study
We aimed to develop a cost-effective and robust method to predict drug resistance in individualpatients. Representative tissue fragments were obtained from tumors removed from femalepatients, aged 24-74 years old. The tumor tissue was taken by a histopathology’s or a surgeonunder sterile conditions. Cells obtained by enzymatic dissociation from tumor after surgery, werecultured as a monolayer f...
متن کاملEffect of Chemotherapeutic Drugs on Caspase-3 Activity, as a Key Biomarker for Apoptosis in Ovarian Tumor Cell Cultured as Monolayer. A Pilot Study
We aimed to develop a cost-effective and robust method to predict drug resistance in individualpatients. Representative tissue fragments were obtained from tumors removed from femalepatients, aged 24-74 years old. The tumor tissue was taken by a histopathology’s or a surgeonunder sterile conditions. Cells obtained by enzymatic dissociation from tumor after surgery, werecultured as a monolayer f...
متن کاملThe Effect of Diazinon on Cell Proliferation and Apoptosis in Testicular Tissue of Rats and The Protective Effect of Vitamin E
Objective Diazinon is an organophosphate pesticide and today this pesticide is mostly used in agriculture. In this study, we analyzed the effects of diazinon and vitamin E on apoptosis and the proliferation of germ cells in rat testis. MaterialsAndMethods In this experimental study 30 male wistar rats, were divided into five groups (N=6). Control, sham (received olive oil), experimental group 1...
متن کاملMesenchymal Stem Cells Derived from Rat Epicardial Versus Epididymal Adipose Tissue
Objective(s) Some investigation has indicated that adipose-derived stem cells possess different surface epitopes and differentiation potential according to the localization of fat pad from which the cells were derived. In the present study proliferation capacity and aging of such cells were explored. Materials and Methods Adherent cells were isolated from the collagenase digests of adipose tiss...
متن کاملبررسی آثار کامپوزیت ژلاتین– کیتوسان بر کشت سلولهای استرومایی مغز استخوان موش صحرایی
Introduction: Gelatin and Chitosan are known as biodegradable and biocompatible biopolymers. These biopolymers have recently received increasingly more attention for tissue engineering. The aim of this study was to survey of effects of Gelatin-Chitosan film in viability, proliferation, apoptosis and differentiation on bone marrow stromal cell (BMSCs) culture in rat. Methods: Fist, gelatin- chi...
متن کامل